Residual dipolar couplings: are multiple independent alignments always possible?
نویسندگان
چکیده
منابع مشابه
Residual dipolar couplings: are multiple independent alignments always possible?
RDCs for the 14 kDa protein hen egg-white lysozyme (HEWL) have been measured in eight different alignment media. The elongated shape and strongly positively charged surface of HEWL appear to limit the protein to four main alignment orientations. Furthermore, low levels of alignment and the protein's interaction with some alignment media increases the experimental error. Together with heterogene...
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Natively unfolded regions have been shown to be common in functional proteins in a vast range of biochemical processes. In contrast to classical structural biology, unfolded proteins must be described by an ensemble of intermediate conformers. The traditional NMR approach, which has been widely used for structure determination in classical structural biology, is still feasible for the study of ...
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Residual dipolar couplings measured in weakly aligning liquid-crystalline solvent contain valuable information on the structure of biomolecules in solution. Here we demonstrate that dipolar couplings (DCs) can be used to derive a comprehensive set of pairwise angular restraints that do not depend on the orientation of the alignment tensor principal axes. These restraints can be used to assess t...
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ژورنال
عنوان ژورنال: Journal of Biomolecular NMR
سال: 2010
ISSN: 0925-2738,1573-5001
DOI: 10.1007/s10858-010-9457-1